| In vitro Motility Assay |
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| 1. Attach myosin S1 on the cover slip |
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| 2. Add fluorescently tagged actin filament |
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| 3. Addition of ATP initiates the movement of
the filaments |
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| 4. Also done by coating cover slip with
actin filaments and use |
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| fluorescently
tagged myosin motor domain |
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