Cells, Organelles: Histochemistry

#103a Kidney. Guinea Pig. Periodic acid-Schiff (PAS) reaction and hematoxylin

Carbohydrates, e.g., glycogen, proteoglycans, glycoproteins, glycosaminoglycans, are demonstrated by this histochemical reaction (see description PAS on p. 110 and in textbooks). Their presence is indicated by a red reaction product. Such structures are termed PAS positive. Note the diffuse reaction at the apical surface of the tubule cells and the more intense reaction along the base of the same cells. What structures are seen with the electron microscope in these stained areas? Nuclei are stained blue because the section was counterstained with hematoxylin.

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